Characterization and in vitro bioactivity of natural hydroxyapatite based bio-glass-ceramics synthesized by thermal plasma processing

Natural bovine hydroxyapatite/SiO2–CaO–MgO glass–ceramics were produced using the transferred arc plasma (TAP) processing method. Homogeneous mixtures of HA/25 wt% SiO2–CaO–MgO and HA/50 wt% SiO2–CaO–MgO batches obtained by dry mixing the respective compositions in a ball mill were processed in argon plasma using the TAP torch at 5 kW for 1, 2 and 3 min, respectively. The synthesized glass–ceramic samples were studied for phase composition, microstructure and bioactivity. The phase study of the synthesized glass–ceramics revealed the formation of calcium phosphate silicate with traces of calcium silicate. The structural study by SEM revealed that the prepared samples possessed smooth glassy surface morphology. The in vitro-bioactivity of the TAP synthesized glass–ceramics was examined in simulated body fluid (SBF). The SBF test results confirmed the development of crystalline carbonated apatite phase after 12 days of immersion. The cytocompatibility was evaluated through human fibroblast cell proliferation. The fibroblasts culture results showed that the sample was non- toxic and promoted cell growth.