Distinct promoters, subjected to epigenetic regulation, drive the expression of two clusterin mRNAs in prostate cancer cells
Articolo
Data di Pubblicazione:
2015
Citazione:
Distinct promoters, subjected to epigenetic regulation, drive the expression of two clusterin mRNAs in prostate cancer cells / Bonacini, M., Coletta, M., Ramazzina, I., Naponelli, V., Modernelli, A., Davalli, P., Bettuzzi, S., Rizzi, F.. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - STAMPA. - 1849:1(2015), pp. 44-54. [10.1016/j.bbagrm.2014.11.003]
Abstract:
The human clusterin (CLU) gene codes for several mRNAs characterized by different sequences at their 5' end. We investigated the expression of two CLU mRNAs, called CLU 1 and CLU 2, in immortalized (PNT1a) and tumorigenic (PC3 and DU145) prostate epithelial cells, as well as in normal fetal fibroblasts (WI38) following the administration of the epigenetic drugs 5-aza-2'-deoxycytidine (AZDC) and trichostatin A (TSA) given either as single or combined treatment (AZDC-TSA). Our experimental evidences show that: In conclusion, we show for the first time that the expression of CLU 2 mRNA is driven by a novel promoter, P2, whose activity responds to epigenetic drugs treatment through changes in histone modifications.
Tipologia CRIS:
Articolo su rivista
Keywords:
Alternative promoter; Clusterin; Epigenetic regulation; Prostate cancer
Elenco autori:
Bonacini, Martina; Coletta, Mariangela; Ramazzina, Ileana; Naponelli, Valeria; Modernelli, Alice; Davalli, Pierpaola; Bettuzzi, Saverio; Rizzi, Federica
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