Proteomic analisys of protein extraction during hemofiltration with on-line endogenous reinfusion (HFR) using different polysulphone membranes

In end-stage renal disease patients, extracor- poreal dialytic therapy is not able to prevent the accumu- lation of toxins related to the uremic syndrome, a severe complication that increases morbidity and mortality rate. In this paper, hemoFiltration with on-line Reinfusion (HFR) architecture is used to evaluate the effect of a more per- meable membrane on the extraction of medium–high molecular weight molecules. The aim of this study was to compare two polysulphone membranes for convective chamber: polyphenylene High Flux (pHF) and polyphen- ylene Super High-Flux (pSHF). Fourteen patients were subjected to HFR with pHF and pSHF membranes and ultra filtrate (UF) samples were collected to evaluate molecular weight cut-off (MWCO) and to identify extracted proteins. Furthermore, image analysis software was used in order to evaluate change in protein extraction during the dialysis. The quantification of four proteins by immunoassay dem- onstrates a higher permeability of pSHF membrane. Two- dimensional electrophoresis (2-DE) gels showed, for both Electronic supplementary material The online version of this article (doi:10.1007/s10856-014-5290-5) contains supplementary material, which is available to authorized users. M. Emanuela (&) ? C. Aurora ? B. Elisa ? B. Stefania ? T. Aldo Department of Diagnostic, Clinical and Public Health Medicine, University of Modena and Reggio Emilia, Via del Pozzo, 71-41124 Modena, Italy e-mail: emanuela.monari@unimore.it C. Marialuisa ? C. Luca ? P. Giuseppe ? S. Luisa ? A. Mauro Scientific Affairs, Bellco s.r.l, Mirandola, Modena, Italy A. Filippo IRCCS Hospital CSS, San Giovanni Rotondo, Foggia, Italy L. Carmelo Division of Nephrology and Dialysis, Legnago, Verona, Italy membranes, the greater number of protein spots at 235 min. Some of the identified proteins, involved in nephropathic disease complications, were compared to assess differences in extraction during dialytic treatment by PDQuest analysis. UF proteomic analysis demonstrated a different behavior for the two membranes; pHF membrane was more permeable at the beginning of HFR treatment (15 min), while pSHF membrane at the end of treatment (235 min). Proteomic analysis is a suitable approach to investigate the behavior of different membranes during dialysis. Results indicated that pSHF membrane offers the higher permeability, and showed higher efficiency in removal of middle molecules related to uremic syndrome.