Publication Date:
2021
Short description:
Analytical Methodologies for Lipidomics in Hemp Plant / Cerrato, A., Capriotti, A.L., Montone, C.M., Aita, S.E., Cannazza, G., Citti, C., Piovesana, S., Aldo, L. (METHODS IN MOLECULAR BIOLOGY). - In: Mass Spectrometry-Based Lipidomics. Methods and Protocols999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA : Humana Press Inc., 2021. - ISBN 978-1-0716-1409-9. - pp. 257-273 [10.1007/978-1-0716-1410-5_17]
abstract:
The chemical composition of Cannabis sativa L. has been extensively studied for tens of years, but little is known about its lipidome. This chapter describes an analytical workflow for polar lipid determination in hemp. After extraction, lipids are enriched and isolated by graphitized carbon black sorbent, and the isolated lipid is analyzed by liquid chromatography (LC) coupled with high resolution mass spectrometry, leading to identification of many lipid species. We have developed a semi-automated platform using commercially available Lipostar software for lipid identification. Our approach affords the identification of 189 polar lipids in hemp extract, including sulfolipids and phospholipids. The number of the identified lipid species is by far the highest ever reported for Cannabis sativa.
Iris type:
Capitolo/Saggio
Keywords:
Cannabis sativa; Graphitized carbon black; Lipidomics; Lipostar; Phospholipids; Sulfolipids
List of contributors:
Cerrato, A.; Capriotti, A. L.; Montone, C. M.; Aita, S. E.; Cannazza, G.; Citti, C.; Piovesana, S.; Aldo, L.
Book title:
Mass Spectrometry-Based Lipidomics. Methods and Protocols
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