Asymmetric reduction of (4S)-(+)-carvone catalyzed by enoate reductases (ERs) expressed by non-conventional yeast (NCY) whole-cells
Chapter
Publication Date:
2012
Short description:
Asymmetric reduction of (4S)-(+)-carvone catalyzed by enoate reductases (ERs) expressed by non-conventional yeast (NCY) whole-cells / P., B., M., G., C., P., E., C., E., B., Cramarossa, M.R., B., T., Forti, L. - In: Practical Methods for Biocatalysis and Biotransformations 2 / J. Whittal and P. Sutton Eds. - STAMPA. - Chichester : John Wiley & Sons, Ltd, 2012. - ISBN 9781119991397. - pp. 104-108
abstract:
(4S)-(þ)-Carvone was totally reduced after 120 h by ER activity expressed by lyophilized whole cells of K. exigua DBVPG 7133 to (1S,4S)-dihydrocarvone (yield¼60%) and (1R,4S)-dihydrocarvone (40%). About 15% of (1S,4S)-dihydrocarvone was further reduced
to (1S,2S,4S)-dihydrocarveol (7%) and (1S,2R,4S)-dihydrocarveol (8%).
In close analogy with previous literature, the use of lyophilized yeast whole cells was generally preferred to growing and resting ones. Besides, because yeast cells contain only catalytic amount of NAD(P)H and cofactor regeneration must take place by means of metabolism of an electron donor, the simultaneous use of lyophilized yeast whole cells and glucose (the last acting as auxiliary substrate for cofactor-recycling systems) showed a significant increase of efficiency in reducing (4S)-(þ)-carvone.
to (1S,2S,4S)-dihydrocarveol (7%) and (1S,2R,4S)-dihydrocarveol (8%).
In close analogy with previous literature, the use of lyophilized yeast whole cells was generally preferred to growing and resting ones. Besides, because yeast cells contain only catalytic amount of NAD(P)H and cofactor regeneration must take place by means of metabolism of an electron donor, the simultaneous use of lyophilized yeast whole cells and glucose (the last acting as auxiliary substrate for cofactor-recycling systems) showed a significant increase of efficiency in reducing (4S)-(þ)-carvone.
Iris type:
Capitolo/Saggio
Keywords:
Asymmetric Reduction; Enoate Reductases; Non-Conventional Yeast
List of contributors:
P., Buzzini; M., Goretti; C., Ponzoni; E., Caselli; E., Branda; Cramarossa, Maria Rita; B., Turchetti; Forti, Luca
Book title:
Practical Methods for Biocatalysis and Biotransformations 2