Biotechnology for health is a rapidly evolving field, with applications ranging from diagnosis to treatment of numerous complex diseases. In particular, the use of advanced biotechnological models and applications has led to significant advances in the understanding and treatment of neurodegenerative and neurological diseases, muscular dystrophies, metabolic diseases, and tumors, as well as in tissue regeneration. Neurodegenerative and neurological diseases can be characterized by the progressive loss of neurons and cognitive functions. Biotechnology offers new hope through the development of cellular and tissue models that allow the study of pathogenic mechanisms and the testing of potential innovative therapies. Muscular dystrophies, such as limb-girdle muscular dystrophy, are rare genetic myopathies that cause progressive muscle weakness and degeneration. Tissue engineering is opening up new avenues for muscle regeneration and the treatment of these diseases. Metabolic diseases, such as diabetes and dyslipidemia, require innovative approaches to diagnosis and treatment. Biotechnology is developing new screening platforms and targeted therapies to address these complex diseases. Finally, cancer represents one of the major challenges to global health. Biotechnology is contributing significantly to the understanding of the molecular mechanisms underlying tumorigenesis and the discovery of new therapeutic targets, improving strategies for cancer prevention, diagnosis, and treatment. The project aims to develop and apply advanced biotechnology strategies to improve the understanding and treatment of neurodegenerative diseases, muscular dystrophies, metabolic diseases, and tumors, as well as to promote tissue regeneration. In particular, the project aims to: 1. Study the pathogenic mechanisms of neurodegenerative and neurological diseases and test potential innovative therapies based on RNA molecules and recombinant therapeutic proteins. 2. Develop tissue engineering approaches for muscle and bone regeneration. 3. Develop new screening platforms and targeted therapies for complex metabolic diseases. 4. Contribute to the understanding of the molecular mechanisms of tumorigenesis and the discovery of new molecular targets to improve cancer therapeutic strategies.