Instrumentation and calibration protocol for a continuous wave near infrared hemoximeter

Quantification of hemoglobin content in vivo using continuous wave (CW) near infrared spectroscopy requires an accurate calibration of the measuring system. The authors introduce a recently developed instrument and focus their attention on the calibration issue, proposing a calibration procedure specifically designed for their system, but which can be easily generalized for other CW near-infrared systems. In the paper, the most important calibration procedures and the results obtained are discussed in detail.