DETERMINATION OF DRUG - MACROMOLECULE BINDING PARAMETERS BY NUMERICAL-ANALYSIS

A method is described for calculating the binding parameters of a drug to a macromolecule from data obtained by indirect techniques, such as spectroscopy, assuming the existence of only one class of binding sites. A critical evaluation of the performance of the method is presented, showing that the errors which previously reported methods may produce are avoided. Some examples that make use of experimental data determined for phenylbutazone and oxyphenbutazone by means of UV difference spectroscopy are given.