Tetracycline Residues in Bovine Muscle and Liver Samples from Sicily (Southern Italy) by LC-MS/MS Method: A Six-Year Study
Articolo
Data di Pubblicazione:
2019
Citazione:
Tetracycline Residues in Bovine Muscle and Liver Samples from Sicily (Southern Italy) by LC-MS/MS Method: A Six-Year Study / Cammilleri, G., Pulvirenti, A., Vella, A., Macaluso, A., Lo Dico, G., Giaccone, V., Giordano, V., Vinciguerra, M., Cicero, N., Cicero, A., Giangrosso, G., Vullo, S., Ferrantelli, V.. - In: MOLECULES. - ISSN 1420-3049. - 24:4(2019), pp. 695-704. [10.3390/molecules24040695]
Abstract:
We examined a total of 369 bovine liver and muscle samples for the detection of
oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), and doxycycline (DOX) residues by
implementation and validation of a LC-MS/MS method. The method showed good recovery values
between 86% and 92% at three levels of concentrations. The linearity tests revealed r2 > 0.996 for all
the tetracyclines examined. Furthermore, the Youden test revealed that the method was robust. Only
14.4% of the samples showed OTC and TC residues in a concentration range of 10.4–40.2 g kg1. No
CTC and DOX residues were found in all the samples analyzed. Liver samples showed the highest
average values (31.5 20.6 and 21.8 18.9 for OTC and TC, respectively). The results showed a
low incidence of TCs in all the samples examined, in comparison with other studies reported in the
literature. A significant decrease in TC residues frequency was found from 2013 (p < 0.05). This work
reports for the first time epidemiological data on the presence of TC residues in liver and muscle
samples of cattle farmed in Sicily (Southern Italy). The very low incidence of TC residues indicates
a continuous improvement in farming techniques in Southern Italy, which is essential to ensure
consumers’ protection.
oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), and doxycycline (DOX) residues by
implementation and validation of a LC-MS/MS method. The method showed good recovery values
between 86% and 92% at three levels of concentrations. The linearity tests revealed r2 > 0.996 for all
the tetracyclines examined. Furthermore, the Youden test revealed that the method was robust. Only
14.4% of the samples showed OTC and TC residues in a concentration range of 10.4–40.2 g kg1. No
CTC and DOX residues were found in all the samples analyzed. Liver samples showed the highest
average values (31.5 20.6 and 21.8 18.9 for OTC and TC, respectively). The results showed a
low incidence of TCs in all the samples examined, in comparison with other studies reported in the
literature. A significant decrease in TC residues frequency was found from 2013 (p < 0.05). This work
reports for the first time epidemiological data on the presence of TC residues in liver and muscle
samples of cattle farmed in Sicily (Southern Italy). The very low incidence of TC residues indicates
a continuous improvement in farming techniques in Southern Italy, which is essential to ensure
consumers’ protection.
Tipologia CRIS:
Articolo su rivista
Keywords:
LC-MS/MS; veterinary drugs; tetracycline; risk assessment; validation process; bovine samples
Elenco autori:
Cammilleri, Gaetano; Pulvirenti, Andrea; Vella, Antonio; Macaluso, Andrea; Lo Dico, Gianluigi; Giaccone, Vita; Giordano, Vincenzo; Vinciguerra, Massimo; Cicero, Nicola; Cicero, Antonello; Giangrosso, Giuseppe; Vullo, Stefano; Ferrantelli, Vincenzo
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