Simulation of optical path and volume-under-test calculation in an integrated dynamic light scattering and autofluorescence device

We have developed a non-invasive diagnostic instrument based on a scanning fluorophotometer integrating autofluorescence and dynamic light scattering techniques. The device makes a scan along the optical axis of the eye. In a DLS measurement, it is extremely important to precisely define the position of the volume-under-test inside the eye and its effective volume. In this work, a specific optical computer aided design tool based on the Navarro's model of the human eye is used to simulate the optical path of the DLS laser beams and to calculate position and size of the volume-under-test.